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2 edition of RNA polymerase of Bacillus subtilis found in the catalog.

RNA polymerase of Bacillus subtilis

Randell T. Libby

RNA polymerase of Bacillus subtilis

regulation, assembly and relative rates of subunit biosynthesis : by Randell T. Libby.

by Randell T. Libby

  • 103 Want to read
  • 5 Currently reading

Published .
Written in English

    Subjects:
  • Bacillus subtilis.

  • The Physical Object
    Pagination[12] 100 leaves, bound :
    Number of Pages100
    ID Numbers
    Open LibraryOL14214506M

      Heterogeneity of RNA polymerase in Bacillus subtilis: evidence for an additional sigma factor in vegetative cells. Proc Natl Acad Sci U S A. May; 78 (5)– [PMC free article] Moran CP, Jr, Losick R, Sonenshein AL. Identification of a sporulation locus in cloned Bacillus subtilis deoxyribonucleic acid. J Bacteriol. Bacteria containing reporter genes that are fused to promoters that respond to antibiotic-induced stress are becoming increasingly popular for screening and characterization of inhibitors during the process of antibacterial drug discovery ().A particularly useful set of strains has been developed using Bacillus subtilis 1S34 as the host. This set is based upon five promoter-luciferase reporter.

    Abstract. Gene 28 of Bacillus subtilis bacteriophage SPO1 codes for a regulatory protein, a sigma factor known as sigma gp28, that binds to the bacterial core RNA polymerase to direct the recognition of phage middle gene promoters. middle promoters exhibit distinctive and conserved nucleotide sequences in two regions centered about 10 and 35 base pairs upstream from the start point of mRNA. Bacillus subtilis RNA polymerase-promoter interactions leading to transcription initiation. The Alu , a B. subtilis bacteriophage dependent on the curved DNA located immediately upstream of the region, and modified forms of Alu were used. Promoters were examined using gel retardation and DNase I footprinting assays.

    The levanase operon of Bacillus subtilis is controlled by RNA polymerase associated with sigma 54 factor and by the LevR activator that is homologous to the NifA/NtrC family of regulators. A ", " promoter is present at the appropriate distance from the transcription start site. The drastic dow . Abstract RNA polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating Bacillus subtilis with antiserum prepared against vegetative core polymerase. The precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.


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RNA polymerase of Bacillus subtilis by Randell T. Libby Download PDF EPUB FB2

It discusses the sporulation, defective bacteriophage, and transformation of Bacillus subtilis. Organized into 11 chapters, the book begins with the genetic map of Bacillus subtilis, followed by DNA replication and RNA polymerase of the said species.

The book then describes the translational apparatus of Bacillus subtilis. RNA polymerase (RNAP) acts at a critical juncture to link the genomic potential of an organism to the expression of this potential as RNA, protein, and ultimately catalytic, structural, and behavioral properties.

Studies in Bacillus subtilis revealed the importance of the conserved TG in the promoter region, as subsequently found in theCited by: Emphasis is placed on comparing the roles of these factors in B.

subtilis RNA polymerase of Bacillus subtilis book gram-negative bacteria. The nusA product from E. coli, associates with RNA polymerase after initiation of transcription and remains associated with the elongation complex.

The sigma subunit of RNA polymerase determines the specificity of promoter by: K.M. Tatti and C.P. Moran, Jr., RNA Polymerase Sigma Factors of Bacillus subtilis: Purification and Characterization.

RNA Polymerase and Associated Factors from Eukaryotes: R.G. Roeder, Nuclear RNA Polymerases: Role of General Initiation Factors and Cofactors in Eukaryotic Transcription. Book chapter Full text access Biochemical Approaches to the Study of Regulatory Modifications of RNA Polymerase; Identification and Characterization of B.

subtilis Sigma 28 RNA Polymerase, an Enzyme of Unique Promoter Specificity M. Chamberlin, M. Gilman. Bacillus subtilis has become widely adopted as a model organism for laboratory studies and is one of the best understood prokaryotes in terms of molecular and cellular biology.

Its superb genetic amenability and relatively large size have provided powerful tools to investigate a bacterium in all possible aspects. This valuable reference work provides a comprehensive and up-to-date analysis of. The chapter focuses on techniques developed for the purification of RNAP and its associated factors from the model gram-positive bacterium Bacillus subtilis.

Bacillus subtilis RNAP has a complex subunit structure. The minimal catalytic moiety, consisting of the ββ´α 2 complex, is associated with two ω subunits to generate the core enzyme.

Methods are also summarized. Purification of Bacillus subtilis RNA Polymerase with Heparin-Agarose. Purification of Bacillus subtilis RNA Polymerase with Heparin-Agarose. IN VITRO TRANSCRIPTION OF DNA*. (Received for publication, Ma ) Barry L. Davison,+ Terrance Leighton,@ and Jesse C. RabinowitzS.

Bacillus subtilis has been one of the principal organisms used for the investigation of genetic transformation. A great deal is known, therefore, about this process (for a review see Dubnau, ).Because the transformation of competent cells by plasmid DNA continues to be the most widely used means of introducing recombinant DNA, we shall discuss this process in some detail later.

The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus.

The interaction of Bacillus subtilis rA with RNA polymerase Elecia B. Johnston, Peter J. Lewis, and Renate Griffith* Discipline of Biological Sciences, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia Received 6 July ; Revised 20 August ; Accepted 21 August DOI: /pro The main early and late promoters of Bacillus subtilis phage phi 29 form unstable open complexes with sigma A-RNA polymerase that are stabilized by DNA supercoiling.

Nucleic Acids. These conserved regions define the affinity of the RNA polymerase complex for a promoter and the accuracy of gene expression. The aim of this paper was to study the promoter regions of Bacillus subtilis bacteria and to make a promoter data set available.

Solution structure of the N-terminal domain of Bacillus subtilis δ subunit of RNA polymerase and its classification based on structural homologs. Proteins: Structure, Function, and Bioinformatics47. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

This sigma factor is responsible for the expression of sporulation specific genes (PubMed). Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined.

Abstract. Low concentrations of the RNA polymerase inhibitor rifampin added to an exponentially growing culture of Bacillus subtilis led to an instant inhibition of growth.

Survival experiments revealed that during the growth arrest the cells became tolerant to the antibiotic and the culture was able to resume growth some time after rifampin treatment.

Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene. Gene98 (1), DOI: /(91)P. George E. Wright, Neal C. Brown. Deoxyribonucleotide analogs as inhibitors and substrates of DNA polymerases.

To study the induction of specific σ factor(s) and the control of the transcription of viral DNA after infection of B. subtilis with ϕ29, we purified the RNA polymerase from uninfected cells and.

A modified form of Bacillus subtilis RNA polymerase (RNA nucleotidyltransferase) has been isolated that exhibits distinctive transcriptional specificity. This modified enzyme transcribes two cloned genes from the purA-cysA region of the B. subtilis chromosome whose expression in vivo is associated with the process of sporulation.

Neither of these genes is transcribed by the usual form of B. Adaptation of bacterial cells to diverse habitats relies on the ability of RNA polymerase to respond to various regulatory signals. Some of these signals are conserved throughout evolution, whereas others are species specific.

In this study we present a comprehensive comparative analysis of RNA polymerases from two distantly related bacterial species, Escherichia coli and Bacillus subtilis.

The Mfd protein is a sequence-independent DNA binding protein with weak ATPase activity. The Mfd protein was able to displace in vitro B. subtilis or E. coli RNA polymerase stalled at a lesion. Therefore, Mfd protein appears to target the transcribed strand for repair by recognizing a stalled RNA polymerase and dissociating it from the DNA.Bacillus subtilis (strain ) Status.

Reviewed-Annotation score: Experimental evidence at DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.Bacillus subtilis (strain ) Status. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

This sigma factor is responsible for the expression of sporulation specific genes. Regions. Feature key.